![]() ![]() CRF was evaluated by serum blood urea nitrogen (BUN) level and creatinine measurements. Male Wistar albino rats were randomly assigned to either the CRF group or the sham-operated control group, which had received saline or melatonin (10 mg/kg, i.p.) for 4 wk. The aim of this study was to examine the role of melatonin in protecting the aorta, heart, corpus cavernosum, lung, diaphragm, and kidney tissues against oxidative damage in a rat model of CRF, which was induced by five of six nephrectomy. Melatonin, the chief secretory product of the pineal gland, was recently found to be a potent free radical scavenger and antioxidant. Considering the decrease in oxidative stress and the intensity of cholestasis, these findings have interesting clinical implications for melatonin as a possible therapeutic agent in biliary cholestasis and parenchymatous liver injury.Ībstract: Chronic renal failure (CRF) is associated with oxidative stress that promotes production of reactive oxygen species (ROS). Finally, melatonin is far more potent and provides superior protection as compared to S-AMe. Third, the results confirm the function of S-AMe as an antioxidant and hepatoprotector. Second, the participation of free radicals of oxygen in the pathogenecity and severity of cholestasis produced by the acute obstruction of the extra-hepatic biliary duct is likely. First, the LDB models cause marked hepatic oxidative stress. ![]() The obtained data permit the following conclusions. However, S-AMe was effective in preventing the loss of GSH in erythrocytes and hepatic tissue, as was melatonin. S-AMe did not modify the increased GGT activity nor did it decrease greatly the TB levels (43% melatonin vs. ![]() Both melatonin and S-AMe administration were effective as antioxidants and hepatoprotective substances, although the protective effects of melatonin were superior it prevented the GSH decrease and reduced significantly the increases in enzyme activities and lipid peroxidation products produced by biliary ligature. At the same time, this procedure produced a notable decrease in the GSH pools in these biological media. LDB caused highly significant increases in plasma enzyme activities and in bilirubin and lipid peroxides levels in erythrocytes and hepatic tissue. Either melatonin or S-AMe were administered daily 3 days before LBD, and for 10 days after biliary obstruction. The severity of the cholestasis and hepatic injury were determined by the changes in the plasma enzyme activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AP), g-glutamyl-transpeptidase (GGT), and levels of albumin, total bilirubin (TB) and direct bilirubin (DB). Hepatic oxidative stress was evaluated by the changes in the amount of lipid peroxides and by the reduced glutathione content (GSH) in lysates of erythrocytes and homogenates of hepatic tissue. In the present research, we studied the effect of the administration of melatonin or S-adenosyl-l-methionine (S-AMe) on oxidative stress and hepatic cholestasis produced by double ligature of the extra-hepatic biliary duct (LBD) in adult male Wistar rats. ![]()
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